Projects/Diffusion/Contrasting Tractography Measures/Slicer Tractography

Whole Brain Seeding

Whole brain seeding followed by selection between two ROIs. Using eddy-corrected data set, without downsampling.

Tensor estimation in DTMRI module in Slicer 2.8. The automatic brain mask generated in conversion step is used to prevent tracking in ventricles and outside the brain.

Tracts are seeded at a resolution of 2mm at points where cL > 0.3; tracking stops when cL drops below 0.15. Tracts shorter than 20mm are discarded. Remaining tracts are saved to disk as separate .vtk files.

Tract selection

Using LaurenThesis module in Slicer 2.8, tracts are selected that pass through 2 specified ROIs.

For all cases of the arcuate fasciculus, none of the tracts generated were found to connect the two ROIs along the arcuate pathway.

Measurement

For all cases, mean scalar measures (FA, mode, trace, linear, planar, spherical) were computed along each individual tract, then combined into a single average measure for each group of tracts. Measurements were done using a Matlab script.

For the cingulum, the three different groups of tracts for each side (L,R) were combined for a single mean measure (note that not all 3 groups were found for every case).

For the uncinate, internal capsule, and fornix, another matlab script was used to cut the tracts where they passed through the ROIs, so that the final measures included only the region of the tracts between the two ROIs. (The pictures below are positioned to show the regions measured; e.g., in the IC pictures, the tracts were measured between the two coronal slices which held the ROIs).

Case	Uncinate Fasciculus	Internal Capsule	Fornix	Cingulum (Left)	Cingulum (Right)
caseD00917
caseD00920
caseD00924
caseD00925			No tracts selected
caseD00928
caseD00935
caseD00936			No tracts selected
caseD00938
caseD00939
caseD00940