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| | 1. Consultation: new request: | | 1. Consultation: new request: |
| − | My dissertation is researching the back of the normal eye. Would you recommend where I could use a microscope that has X-Y automatic platform so that I could use tiling to make a composite picture of the entire retina or eye-cup flatmount? The blood vessels are stained fluorescent green (FITC), the cells are fluorescent red (Alexa 568) and the nuclei infared (TOPRO-3). So far I have been able to image them with an old Leica Confocal using a Z-stack of 25 - 30 um thickness for a flatmount of just the retinal, 50 um for the whole eye-cup with optic nerve attached. 25X immersion objective had high resolution and a long working distance that allowed visualization of the blood vessels wrapped around the optic nerve. 40X - 63X distinguish nuclei of these irregularly shaped cells from high background around the optic disc.
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| − | My preliminary test run with deconvolution in OpenLab on images made on our regular fluorescent microscope, a Leica DM-RXA, may mean that it is not be necessary that the microscope be confocal, as long as it has an automated Z control. On another note, would 3-D Slicer be useful for reconstructing an image of the back of the eye from a combination of confocal microscope z-stack images and OCT (sonic-based imaging)?
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| | 2. Education program:<br> | | 2. Education program:<br> |
Revision as of 17:19, 30 March 2010
Home < CTSC:TTIC.033010Back to Collaboration:Harvard_CTSC
1. Consultation: new request:
2. Education program:
a. update on 2nd cardiovascular session and 1st oncology session
b. policy for file sharing
c. New material for hands-on workshops
3. RSNA education proposal and reading room of the future
4. Multi-center trial
