Projects:RegistrationLibrary:RegLib C03

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Slicer Registration Library Exampe #3: Diffusion Weighted Image Volume: align with structural reference MRI

Input

fixed image/target T1 SPGR		moving image 1 T2		moving image 2a DTI baseline	moving image 2b DTI tensor

Modules

• Slicer 3.6.1 recommended modules: BrainsFit

Objective / Background

This is a typical example of DTI processing. Goal is to align the DTI image with a structural scan that provides accuracte anatomical reference. The DTI contains acquisition-related distortion and insufficient contrast to discern anatomical detail.

Keywords

MRI, brain, head, intra-subject, DTI, DWI

Download

• download entire package (Data,Presets, Solution, zip file 24 MB)
• Presets
• Tutorial only
• Image Data only

Link to User Guide: How to Load/Save Registration Parameter Presets

Input Data

• reference/fixed : T2w axial, 0.4mm resolution in plane, 3mm slices
• moving: Baseline image of acquired DTI volume, corresponds to T2w MRI , 0.9375 x 0.9375 x 1.4 mm voxel size, oblique
• tag: Tensor data of DTI volume, oblique, same orientation as Baseline image. The result Xform will be applied to this volume. The original DWI has 26 directions, the extracted DTI volume has 9 scalars, i.e. 256 x 256 x 36 x 9

Procedures

• Phase I: LOAD DATA

1. download example dataset
2. load into 3DSlicer 3.6.1 (Load Scene)
3. To convert the DWI into a DTI: use the Converters / DICOM to NRRD Converter module

• Phase II: Register T2 to T1

1. open Registration : BrainsFit module
   1. Registration Phases:
   2. select/check Include Rigid registration phase
   3. select/check Include Affine registration phase
   4. select a new transform Output Transform
2. Registration Parameters: increase Number Of Samples to 200,000
3. Leave all other settings at default
4. click apply; runtime ca. 1-2 min.
5. Resample T2 into T1 space
   1. Open Resample Scalar/Vector/DWI Volume module (Filtering menu)
   2. Input Volume: T2, Reference Volume: T1
   3. Output Volume: create new volume, rename to "T2_Xf1"
   4. Interpolation Type: select ws (windowed sinc)
   5. Click Apply.
   6. Upon completion, go to Volumes module to adjust window & level
   7. Active Volume: select T2_Xf1
   8. Open Display tab and adjust window & level, e.g. 1300/700

• Phase III:REGISTER DTI TO T2_Xf1

1. open Registration : BrainsFit module
   1. Registration Phases:
   2. set T2_Xf1 as fixed and DTI_baseline as moving image
      1. select/check Include Rigid registration phase
      2. select/check Include Affine registration phase
      3. select/check Include BSpline registration phase
      4. select Include Rigid registration phase
      5. select Include Affine registration phase
      6. select Include BSpline registration phase
   3. Output Settings:
      1. select a new transform "Slicer BSpline Transform", rename to "Xf2_DTI-T1_unmasked"
      2. select a new volume "Output Image Volume, rename to "DT_base_Xf2"
   4. Registration Parameters: increase Number Of Samples to 200,000
   5. Registration Parameters: set Number Of Grid Subdivisions to 5,5,3
   6. Leave all other settings at default
   7. click: Apply; runtime < 1 min.

• Phase IV: Resample DTI_mask
  • we use the above Xform to produce a mask for the T1.

1. Open Resample Scalar/Vector/DWI Volume module
   1. Input Volume: DTI_mask; Output volume: create new volume, rename to "DTI_mask_Xf2"
   2. Transform Node: "Xf2_DTI-T1_unmasked"
   3. select/check: output-to-input
   4. Interpolation Type: select: nn
   5. click: Apply

• Phase V:REGISTER DTI TO T2_Xf1 with masking

1. open Registration : BrainsFit module
   1. Registration Phases: as before: rigid, affine, BSpline
   2. set T2_Xf1 as fixed and DTI_baseline as moving image
   3. All parameters as in Phase III
   4. Output BSpline Transform: create new , rename to "Xf3_DTI-T1_masked"
   5. Output Volume: create new, rename to "DTI_base_Xf3"
   6. Control of Mask Processing
      1. select/check: ROI (rightmost box)
      2. Input Fixed Mask: select "DTI_mask_Xf2"
      3. Input Moving Mask: select "DTI_mask"
   7. click: Apply; runtime < 1 min.

• Phase VI: Resample DTI

1. Load the combined transform (Add Data)
2. Open the Resample DTI Volume module (found under: All Modules)
   1. Input Volume: select DTI
   2. Output Volume: select New DTI Volume, rename to DTI_Xf2
   3. Reference Volume: select T1
   4. Transform Parameters: select transform "Xf2_DTI-T1
   5. check box: output-to-input
   6. Leave all other settings at defaults
   7. Click Apply; runtime < 1 min.
3. Go to the Volumes module, select the newly produced DTI_Xf2 volume
4. under the Display tab, select Color Orientation from the Scalar Mode menu
5. set T1 as background and new DTI_Xf2 volume as foreground
6. Set fade slider to see DTI overlay onto the SPGR image

for more details see the tutorial(s) under Downloads

Registration Results

baseline to T2 after affine alignment

Discussion: Registration Challenges

• The DTI contains acquisition-related distortions (commonly EPI acquisitions) that can make automated registration difficult.
• the two images often have strong differences in voxel sizes and voxel anisotropy. If the orientation of the highest resolution is not the same in both images, finding a good match can be difficult.
• there may be widespread and extensive pathology (e.g stroke, tumor) that might affect the registration if its contrast is different in the baseline and structural reference scan

Discussion: Key Strategies

• the two images have identical contrast, hence we could consider "sharper" cost functions, such as NormCorr or MeanSqrd. But because of the strong distortions and lower resolution of the moving image, Mutual Information is recommended as the most robust metric.
• often anatomical labels are available from the reference scan. It would be less work to align the anatomical reference with the DTI, since that would circumvent having to resample the complex tensor data into a new orientation. However the strong distortions are better addressed by registering the other direction, i.e. move the DTI into the anatomical reference space.
• because we seek to assess/quantify regional size change, we must use a rigid (6DOF) scheme, i.e. we must exclude scaling.
• masking is likely necessary to obtain good results.
• in this example the initial alignment of the two scans is very poor. The strongly oblique orientation of the DTI makes an initial manual alignment step necessary.
• these two images are not too far apart initially, so we reduce the default of expected translational misalignment
• because speed is not that critical, we increase the sampling rate from the default 2% to 15%.
• we also expect larger differences in scale & distortion than with regular structural scane: so we significantly (2x-3x) increase the expected values for scale and skew from the defaults.
• a good affine alignment is important before proceeding to non-rigid alignment to further correct for distortions.