2010 Summer Project Week Primate image analysis using the standard Slicer pipeline

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Home < 2010 Summer Project Week Primate image analysis using the standard Slicer pipeline


Key Investigators

  • UNC: Yundi(Wendy) Shi, Clement Vachet, Martin Styner
  • Emory: Mar Sanchez
  • BWH: Andriy Fedorov

Objective

To build an open-source software (mainly involving Slicer3) based pipeline for primate imaging, including skull stripping of T1,T2 and DTI images, tissue segmentation, cortical thickness analysis, atlas building and fiber tracing and tract-based analysis. This is part of a longitudinal study of rhesus macaque monkeys



Approach, Plan

  • Skull stripping and Tissue segmentation

ABC (developed by Marcel Prastawa, SCI, Utah) is currently used for skull stripping and tissue segmentation of the 3 months, 6 months and 12 months T1 and T2 images. Challenge include: Application of ABC or neoseg for the same task on 2 weeks old monkey images, due to the different anatomical structures during early ages and small brain size of the monkeys

  • Cortical thickness analysis

Will work with Clement Vachet and adapt the tools for human data to primate data

  • Affine alignment

RVIEW is currently used for affine alignment of the monkey images. Working on testing the data with the Slicer tools and replace rview tools with them. We need to work with ?? for the special challenge in registration of the monkey brains.

  • Atlas building

AtlasWerks is the tool for atlas building with fWarp (developed by Sarang Joshi, SCI, UTAH). We would like to start using open source tools like the deamons registration in Slicer for the same task.


Progress

  • Attended the DTI breakout session, and identified the problem/challenge of the commonly used tools in the pipeline used by people in the community and the tools we usually use (DicomtoNrrdConvert...)
  • Work with Hans, and tested BrainsFit for the "affine alignment" task in tissue segmentation, which includes rigid registration of T1 and T2 images, as well as the alignment of subject with template using affine registration. Parameters were selected for monkey data with good results.
  • Testing Demons for warping the subjects to atlas, instead of fWarp.
  • Meeting with Steve for using the color module for visualization of fiber bundles with scalars with custom min and max and workaround to visualize fibers as tubes with scalar values written in the vtk lookup table.

Delivery Mechanism

This work will be delivered as open-source software and open dataset

References

  • M. Styner, R. Knickmeyer, S. Joshi, C. Coe, S. J. Short, and J. Gilmore. Automatic brain segmentation in rhesus monkeys. In Proc SPIE Vol 6512, Medical Imaging, 2007, pp. 65122 L1-8.